| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059521 |
| Chromosome: | chromosome_1 |
| Location: | 3898944 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025400 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGGATGGTGTCCTCGCGGTTTGTGGTGAG |
| Internal bar code: | GAGTCAGTCATGCTCGGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 769 |
| LEAP-Seq percent confirming: | 99.7331 |
| LEAP-Seq n confirming: | 10836 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCTCTAACCTCACCCG |
| Suggested primer 2: | CGGGTTGATGAACTTGAGGT |