| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059539 |
| Chromosome: | chromosome 12 |
| Location: | 8961130 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544114 | CYN20-6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGATGGGCACAGGGCAGCTGCGCAACG |
| Internal bar code: | GGGGGGCTGCTCTAAGGAGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 98.5138 |
| LEAP-Seq n confirming: | 1856 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATTGAACGCTTGTACCT |
| Suggested primer 2: | GGTACTGGCGGTTACAGCAT |