Insertion junction: LMJ.RY0402.059931_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.059931
Chromosome:chromosome 16
Location:7279858
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g683371 ODC2 (1 of 2) 4.1.1.17 - Ornithine decarboxylase / L-ornithine carboxy-lyase; Ornithine decarboxylase 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACTTGAACTCCGGGTCCAACTCCGCAAT
Internal bar code:CCGCTCTTGGGACTCATTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:641
LEAP-Seq percent confirming:32.552
LEAP-Seq n confirming:1236
LEAP-Seq n nonconfirming:2561
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGTGATGTTCACACGACA
Suggested primer 2:CTACGCTGTCAAGGTGCGTA