Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.060962 |
Chromosome: | chromosome 3 |
Location: | 5237700 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g182950 | (1 of 1) K12820 - pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15/PRP43 (DHX15, PRP43) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCTATCTGCATGCCACGTGTTACGGCGT |
Internal bar code: | TGGTGTGTCTCGAGCGCGCACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 873 |
LEAP-Seq percent confirming: | 99.1641 |
LEAP-Seq n confirming: | 8660 |
LEAP-Seq n nonconfirming: | 73 |
LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCCCTAGGTCTGAGCTGTT |
Suggested primer 2: | CCTGTGACCAAAATGACGTG |