Insertion junction: LMJ.RY0402.060962_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.060962
Chromosome:chromosome 3
Location:5237700
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g182950 (1 of 1) K12820 - pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15/PRP43 (DHX15, PRP43) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCTATCTGCATGCCACGTGTTACGGCGT
Internal bar code:TGGTGTGTCTCGAGCGCGCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:873
LEAP-Seq percent confirming:99.1641
LEAP-Seq n confirming:8660
LEAP-Seq n nonconfirming:73
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCCTAGGTCTGAGCTGTT
Suggested primer 2:CCTGTGACCAAAATGACGTG