| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060965 |
| Chromosome: | chromosome 3 |
| Location: | 8059680 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201300 | SSA10 | cilia-sensing, structure and/or assembly; (1 of 1) PTHR10157//PTHR10157:SF27 - DOPAMINE BETA HYDROXYLASE RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGATGCCGCACGATGCCAAGTATCACGT |
| Internal bar code: | AGCCCTGGTTGGCAAAGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 650 |
| LEAP-Seq percent confirming: | 99.6753 |
| LEAP-Seq n confirming: | 1228 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCAACATGAGCCGTTTAG |
| Suggested primer 2: | GCAGAGTTCGGAGTTCAAGG |