| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061213 |
| Chromosome: | chromosome 17 |
| Location: | 2877681 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719500 | AOF9 | (1 of 2) 1.3.99.29 - Phytoene desaturase (zeta-carotene-forming) / 2-step phytoene desaturase; Flavin-containing amine oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATCTGCTTCATCACCACGTCCTCCACC |
| Internal bar code: | GACTCCGTTGTCCCTGTGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 54.6424 |
| LEAP-Seq n confirming: | 4867 |
| LEAP-Seq n nonconfirming: | 4040 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCAGGTGTGGTTAGA |
| Suggested primer 2: | ATGTGTGTGCGTGTCATGTG |