| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061388 |
| Chromosome: | chromosome 16 |
| Location: | 3687572 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688750 | (1 of 1) K13109 - IK cytokine (IK, RED, RER) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAGACAGCGACGAGGAGGATGAGGAGC |
| Internal bar code: | GTTAGGGCCGTCAAGGCGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 98.8212 |
| LEAP-Seq n confirming: | 2012 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCCACTGCATCTCAGCA |
| Suggested primer 2: | GCCGACTGACAAGAAGAAGG |