| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061634 |
| Chromosome: | chromosome 13 |
| Location: | 3148003 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585150 | EIF6A,EIF6 | (1 of 1) K03264 - translation initiation factor 6 (EIF6); Eukaryotic translation initiation factor 6 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTGCCTCATCCTAATTTACGGCCGCCC |
| Internal bar code: | GCGCGGTGACTGGTTGGGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 929 |
| LEAP-Seq percent confirming: | 99.6676 |
| LEAP-Seq n confirming: | 2099 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTCGCAGTTGCCTTAC |
| Suggested primer 2: | CAGTCGTTCGCTACTAGCCC |