| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061918 |
| Chromosome: | chromosome 3 |
| Location: | 6398214 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194200 | PDH2 | Pyruvate dehydrogenase E1 beta subunit; (1 of 1) PTHR11624//PTHR11624:SF76 - DEHYDROGENASE RELATED // PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA-2, CHLOROPLASTIC-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGACCATGCTACTGGTCTCTGTCTCCA |
| Internal bar code: | TTTTGCGTTGGAGTGTCCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 99.6386 |
| LEAP-Seq n confirming: | 827 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACAACTGCTTGTGCTGC |
| Suggested primer 2: | GGGAGCTCAGGGTAGTAGGG |