| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062103 |
| Chromosome: | chromosome 12 |
| Location: | 4801532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524350 | HUS1 | DNA damage checkpoint protein; (1 of 1) K10903 - HUS1 checkpoint protein (HUS1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACATGCTGACTCACCCAGCAGCACCTGC |
| Internal bar code: | CAGTACTTTCGCCGACCTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 96.7554 |
| LEAP-Seq n confirming: | 1163 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAAGCTGGAGAGCTGGA |
| Suggested primer 2: | ACCTGCTGCGCTAGATTCAT |