| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062360 |
| Chromosome: | chromosome 6 |
| Location: | 6670263 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294200 | (1 of 1) 6.5.1.2 - DNA ligase (NAD(+)) / Polynucleotide ligase (NAD+) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTCGTACCTCGAGCGTGCCGGTGACGC |
| Internal bar code: | AAGTCTGGGAACACCCAGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 732 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 426 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGGATGTAAATGACGC |
| Suggested primer 2: | AAATTGAAGTGGAAGTGGCG |