Insertion junction: LMJ.RY0402.062938_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.062938
Chromosome:chromosome_3
Location:7303283
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g206900 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):ACGAGGGCGCATGCCCCAGCAGCGCCGGCA
Internal bar code:CGCTAGTTCACGGAGTTTGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:613
LEAP-Seq percent confirming:98.3871
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGCACTTCCCCAATTTT
Suggested primer 2:CATGGCCTCATCCTACTCGT