Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.062938 |
Chromosome: | chromosome_3 |
Location: | 7303283 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g206900 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACGAGGGCGCATGCCCCAGCAGCGCCGGCA |
Internal bar code: | CGCTAGTTCACGGAGTTTGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 613 |
LEAP-Seq percent confirming: | 98.3871 |
LEAP-Seq n confirming: | 61 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACTGCACTTCCCCAATTTT |
Suggested primer 2: | CATGGCCTCATCCTACTCGT |