Insertion junction: LMJ.RY0402.063018_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.063018
Chromosome:chromosome 11
Location:771468
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467634 (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCAGCGCTCGCCAACTTCACGTCCTGC
Internal bar code:GGTGAGTCGGAGACACCGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:99.0826
LEAP-Seq n confirming:108
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTAGGCTACTGGCCTTG
Suggested primer 2:ACAGCGATCGTGTGAAACTG