| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063057 |
| Chromosome: | chromosome 5 |
| Location: | 585502 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232200 | NDA3,LCI18 | Mitochondrial NADH dehydrogenase; (1 of 1) PTHR22912:SF180 - 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE FERREDOXIN--NAD(+) REDUCTASE COMPONENT | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTTTTGTATAAACTTTTGTTGGCAATT |
| Internal bar code: | CGAAGTTGGGCATTGCGCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 99.7575 |
| LEAP-Seq n confirming: | 4113 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGTTGAGATGATGGGAGC |
| Suggested primer 2: | GTGCTTCCTGGGTCTTACCA |