| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.063216 |
| Chromosome: | chromosome 1 |
| Location: | 3888580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025300 | RAD51B | (1 of 1) K10869 - RAD51-like protein 1 (RAD51L1, RAD51B); DNA recombination protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACAAACATCGCCAGACTTGTTTAGCAG |
| Internal bar code: | GTTCTCATCGCATGTTAATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 99.6066 |
| LEAP-Seq n confirming: | 9369 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACTGCTGGATGGGTAGT |
| Suggested primer 2: | AGGCAGACGACGATTCAAGT |