Insertion junction: LMJ.RY0402.063325_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.063325
Chromosome:chromosome 9
Location:7151335
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g412000 (1 of 26) IPR013026//IPR019734 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTTCTGCTGCCATCTCTGACTTTGACAC
Internal bar code:AGCCGCAGAAATACGGCAAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:483
LEAP-Seq percent confirming:98.9618
LEAP-Seq n confirming:6005
LEAP-Seq n nonconfirming:63
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTCAACACAGCCCCTAAC
Suggested primer 2:GAACTCCATGGCTGACTGGT