| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063450 |
| Chromosome: | chromosome 14 |
| Location: | 1923310 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621000 | SPL27 | Pre-mRNA splicing factor; (1 of 1) K12869 - crooked neck (CRN, CRNKL1, CLF1, SYF3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATCAAGCTGGAGGAGGGCACCGGGGAC |
| Internal bar code: | TTACCCCGCGGGGAGCAAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 99.8397 |
| LEAP-Seq n confirming: | 623 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTCGCTGAGTTCGAGGA |
| Suggested primer 2: | GAAGAAAACAGCCGCGTAAA |