| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064777 |
| Chromosome: | chromosome 9 |
| Location: | 3012160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387400 | POLZ1 | DNA polymerase zeta; (1 of 1) K02350 - DNA polymerase zeta (POLZ1, rev3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGAGGCCCGGGCAAGTGGTGGCGACATT |
| Internal bar code: | CGGACCCGTGTATCCCCTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 99.6448 |
| LEAP-Seq n confirming: | 561 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGACTGCACGTTTGAGG |
| Suggested primer 2: | AAGGTGTCCGGTGATACAGC |