| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065209 |
| Chromosome: | chromosome 13 |
| Location: | 1294661 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571050 | High mobility group protein; (1 of 1) PF00505//PF01388 - HMG (high mobility group) box (HMG_box) // ARID/BRIGHT DNA binding domain (ARID) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCGCCGCCGCAGCGGCTGCAGCCGATG |
| Internal bar code: | ATATTGCAGCTGACCCAGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 83.2574 |
| LEAP-Seq n confirming: | 731 |
| LEAP-Seq n nonconfirming: | 147 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCCATGACGAAGACTCGG |
| Suggested primer 2: | CGGCGCATTTATCTTCTTCT |