Insertion junction: LMJ.RY0402.065353_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.065353
Chromosome:chromosome 13
Location:2213521
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGAAACATTCAAGAAGATATTTGCAGGT
Internal bar code:GGTGTCTTAGGGACATGTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:285
LEAP-Seq percent confirming:99.6026
LEAP-Seq n confirming:5264
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CATCAGGCAAACTCGTGCTA
Suggested primer 2:CGAATACCCAATCATCCAGG