Insertion junction: LMJ.RY0402.065643_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.065643
Chromosome:chromosome 3
Location:1429032
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g151250 LANC1,LAN1 (1 of 1) PF05147 - Lanthionine synthetase C-like protein (LANC_like); LanC lantibiotic synthetase component C-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCATCATGCCGTGCGCCGCGCCCAGGTA
Internal bar code:ACGGATGGACGCACGGATTAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:363
LEAP-Seq percent confirming:56.5957
LEAP-Seq n confirming:133
LEAP-Seq n nonconfirming:102
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTAACGCTTTGTGCT
Suggested primer 2:TTCAGTTGTAAGCGGTGTCG