Insertion junction: LMJ.RY0402.066007_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066007
Chromosome:chromosome 16
Location:5253683
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g682550 (1 of 1) PF08016//PF08263//PF13855 - Polycystin cation channel (PKD_channel) // Leucine rich repeat N-terminal domain (LRRNT_2) // Leucine rich repeat (LRR_8) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGGCAGCGTCCCCGAAACCGCGGGCAG
Internal bar code:CAACAACGTGTCGCAGGTGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:371
LEAP-Seq percent confirming:93.0899
LEAP-Seq n confirming:1657
LEAP-Seq n nonconfirming:123
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGATGAGGGTAGATCGT
Suggested primer 2:CCTCCCGTACCGTCACTCTA