| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066501 |
| Chromosome: | chromosome 7 |
| Location: | 5462791 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350700 | RAA6 | psaA mRNA trans-splicing factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCCACGCCCGTGCTTGTGGTGCCACAG |
| Internal bar code: | CTGGTACTGTTTTGATAATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 98.3389 |
| LEAP-Seq n confirming: | 296 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCGGGATTCCTAAATGT |
| Suggested primer 2: | CGGTGATGAATCATGGTTTG |