| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066580 |
| Chromosome: | chromosome 11 |
| Location: | 3725715 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482850 | (1 of 1) K16812 - targeting protein for Xklp2 (TPX2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACTCTGTCGGCCGTGGACATGACAACC |
| Internal bar code: | CTTCGCACAGCTCCCCAGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 94.9091 |
| LEAP-Seq n confirming: | 261 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGTAGTGCTTTGGATGT |
| Suggested primer 2: | ATTCAGGGGACTACAATGCG |