| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066751 |
| Chromosome: | chromosome 4 |
| Location: | 2660873 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223050 | CAH2 | (1 of 2) PTHR18952:SF104 - CARBONIC ANHYDRASE-RELATED PROTEIN; Carbonic anhydrase, alpha type%252C periplasmic | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTTCGGCGCGATAAAAGCATGCTTCGT |
| Internal bar code: | TATTGCCACGCGGCTTGTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 98.4324 |
| LEAP-Seq n confirming: | 4835 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCACGCTTTTCGTTCTG |
| Suggested primer 2: | AGGCCAGGTCCTTACCAGTT |