Insertion junction: LMJ.RY0402.067168_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.067168
Chromosome:chromosome_3
Location:3046067
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre03.g164250 FAP262 Flagellar Associated Protein, protein kinase sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CGCCTTCGGCGTTGGCGGCAGCGGCGCCGC
Internal bar code:ATCTTATTCACTCATATACCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTACGCATTCAGACACAGGC
Suggested primer 2:GTTCAACACGCAAGCAAGAA