Insertion junction: LMJ.RY0402.067168_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.067168
Chromosome:chromosome 3
Location:3046177
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g164250 FAP262 (1 of 1) PF00612//PF07714 - IQ calmodulin-binding motif (IQ) // Protein tyrosine kinase (Pkinase_Tyr); Flagellar Associated Protein 262, protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGACGCCCACCATCGACGCGCTGGCGGAG
Internal bar code:ATGTCAGCCAAGCGGGGTGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:157
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:157
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTACGCATTCAGACACAGGC
Suggested primer 2:GTTCAACACGCAAGCAAGAA