| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067190 |
| Chromosome: | chromosome_4 |
| Location: | 674437 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g217951 | LCI33 | Low-CO2-inducible protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTGGGCCCGGTTTCTTGCAGCCCGCTTCA |
| Internal bar code: | CTCGAAGTCTTTCGGGCGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1085 |
| LEAP-Seq percent confirming: | 99.7518 |
| LEAP-Seq n confirming: | 31754 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGGCGAGCAAGGTTTAG |
| Suggested primer 2: | AACCTACGGTGATGGTCAGC |