| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067291 |
| Chromosome: | chromosome 9 |
| Location: | 854183 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401700 | (1 of 1) PTHR16193:SF0 - TETRATRICOPEPTIDE REPEAT PROTEIN 27 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCGAGAGACTCAAACACGCGGATGGCA |
| Internal bar code: | GAATACCAAGCGTTACCGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 54.2553 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGAGATACAGGCCAGCG |
| Suggested primer 2: | AGGAGAGGAAGGAGCAGGAG |