| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067427 |
| Chromosome: | chromosome 9 |
| Location: | 679861 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402950 | CGL21 | Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGATTGCAATGAGCTAAGCTGCCCCACG |
| Internal bar code: | GTCCCTGTAATGGTTGGTGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1124 |
| LEAP-Seq percent confirming: | 99.7856 |
| LEAP-Seq n confirming: | 4188 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGAAGAACCCCAACATCA |
| Suggested primer 2: | AAAGCAAAACAAGAGGCGAA |