| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067880 |
| Chromosome: | chromosome 1 |
| Location: | 5379401 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037800 | TRL7,TRX21 | (1 of 11) IPR012336//IPR013766 - Thioredoxin-like fold // Thioredoxin domain; Thioredoxin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGAAGCGCGTGGCTGAAATGAAGAAGC |
| Internal bar code: | CGTTAGTCAACATTCCCTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1100 |
| LEAP-Seq percent confirming: | 99.3959 |
| LEAP-Seq n confirming: | 3949 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTGAGCACGTGAGTATT |
| Suggested primer 2: | TTGGCTACAAAGAAGCAGCA |