| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068123 |
| Chromosome: | chromosome 13 |
| Location: | 1252079 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570550 | CDD1 | Cytidine deaminase; (1 of 1) 3.5.4.5 - Cytidine deaminase / Cytosine nucleoside deaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTATGCCTCCCTCAGCCGCTCACCGTA |
| Internal bar code: | ATTCAGGTCGGGTTAGCGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTACGTGTGTGTGTGGG |
| Suggested primer 2: | GTTGATTGGGTGATGCAGTG |