Insertion junction: LMJ.RY0402.068640_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.068640
Chromosome:chromosome 11
Location:999755
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467665 (1 of 1) IPR002909//IPR006626//IPR011050//IPR014756//IPR019316 - IPT domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Immunoglobulin E-set // G8 domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTGACTAACACCAGCGGACTGGTGCTGG
Internal bar code:CGACCTTGTGTCCTGCTCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:963
LEAP-Seq percent confirming:99.469
LEAP-Seq n confirming:1686
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTACCAGCAATTCATTGGCA
Suggested primer 2:CCGATACCGCTTTTCACCTA