Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.068834 |
Chromosome: | chromosome 5 |
Location: | 431624 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g232751 | (1 of 2) PTHR23518:SF2 - MULTIDRUG RESISTANCE PROTEIN MDTH | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTGAAGCCCCCCTGCCCACCTACCGTA |
Internal bar code: | TTGCCGTTGGGTGGTGACGGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 358 |
LEAP-Seq percent confirming: | 99.0874 |
LEAP-Seq n confirming: | 760 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCCAGGAGCGTAGAGAACG |
Suggested primer 2: | ATTTGTTTCCATGCCAGGAG |