| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.069021 |
| Chromosome: | chromosome 4 |
| Location: | 2450055 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221550 | CVDE1,NPQ1,FAO5,CVDE,VDE | Chlorophycean Violaxanthin De-epoxidase; (1 of 3) 5.5.1.19 - Lycopene beta-cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCGGCTAGTAGTATCGGTGTTGCAAGA |
| Internal bar code: | CACAGGCCTTTTCTAGGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1090 |
| LEAP-Seq percent confirming: | 99.4398 |
| LEAP-Seq n confirming: | 710 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAATGCTGACAGCGAGTA |
| Suggested primer 2: | AACCCACCAACCAACATCAT |