| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069070 |
| Chromosome: | chromosome 4 |
| Location: | 1594017 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213300 | (1 of 88) IPR011333 - POZ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGACCAGGCCACAGCGGACGTGTCGCTCG |
| Internal bar code: | TCACCGTTACACGCAAGCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 70.5236 |
| LEAP-Seq n confirming: | 1347 |
| LEAP-Seq n nonconfirming: | 563 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACACACACAGCCCAAGG |
| Suggested primer 2: | TGCAGTCTTACAGACGCACC |