| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069382 |
| Chromosome: | chromosome_16 |
| Location: | 2596821 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g661626 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGATTTCCTGTCCCGTCAGCCTCTTCTGC |
| Internal bar code: | AACTGTGTGGGCGGCGTCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCCGGGGTATTATGTGG |
| Suggested primer 2: | CACGGCGTTATGTTCAAATG |