| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069660 |
| Chromosome: | chromosome 12 |
| Location: | 7862997 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552900 | DOI1,LIS1 | outer arm dynein interacting protein; (1 of 24) IPR001680//IPR017986//IPR020472 - WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGGGATACCATTCTACACTGGTGTAAT |
| Internal bar code: | CGGGTAGGGCTGCAACGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 99.8302 |
| LEAP-Seq n confirming: | 2352 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAATGGACAACGCTTAGG |
| Suggested primer 2: | GCAGTAAGGCCCCTAGCTCT |