| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069874 |
| Chromosome: | chromosome 1 |
| Location: | 374271 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002300 | CYN4,CYN19B,CYN19-2,ROC1 | (1 of 4) K01802 - peptidylprolyl isomerase (E5.2.1.8); Cyclophilin 19-2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACTTCAAGGGCTCCACCTTCCACCGTGT |
| Internal bar code: | TACCAAAGTGTGAAAATTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 99.7498 |
| LEAP-Seq n confirming: | 5183 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCACAGCGACATAGGTG |
| Suggested primer 2: | GCGACCATCATTACAGGCTT |