Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.069874 |
Chromosome: | chromosome_1 |
Location: | 374271 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g002300 | CYN19-2,CYN4 | Peptidyl-prolyl cis-trans isomerase, cyclophilin-type | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCACTTCAAGGGCTCCACCTTCCACCGTGT |
Internal bar code: | TACCAAAGTGTGAAAATTGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 448 |
LEAP-Seq percent confirming: | 99.7498 |
LEAP-Seq n confirming: | 5183 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGCACAGCGACATAGGTG |
Suggested primer 2: | GCGACCATCATTACAGGCTT |