| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070143 |
| Chromosome: | chromosome 12 |
| Location: | 8147647 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551200 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTGCTGCCTCGGAGCTACCCCTCATGT |
| Internal bar code: | CACCCCACTTCCCATCTTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 95 |
| LEAP-Seq percent confirming: | 94.2792 |
| LEAP-Seq n confirming: | 1648 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGAAGGGCGAACTGCTT |
| Suggested primer 2: | CGCCTGTGTTATGAAGGGAT |