| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070600 |
| Chromosome: | chromosome 9 |
| Location: | 4065886 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393358 | VIG1 | Vasa-intronic gene homolog; (1 of 1) K13199 - plasminogen activator inhibitor 1 RNA-binding protein (SERBP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTAACCATTCCGAGAACCAGGGGCATC |
| Internal bar code: | GCTGCTTCGGGCTGTGCATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 264 |
| LEAP-Seq percent confirming: | 99.5146 |
| LEAP-Seq n confirming: | 205 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAAAGTGCTAGGTAACGC |
| Suggested primer 2: | GCAACGGACTGGTTTTTGAT |