Gene: Cre09.g393358

Overview

Common name:VIG1
Defline:Vasa-intronic gene homolog; (1 of 1) K13199 - plasminogen activator inhibitor 1 RNA-binding protein (SERBP1)
Description:Ortholog of Drosophila VIG gene, required for translational inhibition by RNA-induced silencing complexes formed by sRNA and Argonaute but dispensable for cleavage-mediated RNA interference; localizes to mono- and polyribosomes in the cytosol; copurifies with AGO3 and DCL3; mutant is hypersentitive to cycloheximide
Synonyms:Cre02.g129300.t1.1,g9752.t1,Cre02.g129300.t1.2
Chromosome:chromosome 9

Protein Localization

A plasmid for expressing Cre09.g393358 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre09.g393358-Venus-3xFLAG

Imaged strain: CSI_LW11C8

Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.

Classification(s):

cytosol/cytosol (strong signal)

If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.

VenusChlorophyllBright fieldMerged

Protein-Protein Interactions

We did not determine any protein-protein interactions for this gene.

Mutant Strains

Insertion Junction Mutant Strain Insertion Junction Feature Confidence (%) Genome Version
CLIP2.012818_1 CLIP2.012818 intron 80 6.1
CLIP2.057130_1 CLIP2.057130 3'UTR 80 6.1
CLIP2.057130_2 CLIP2.057130 3'UTR 80 6.1
CLIP2.060682_1 CLIP2.060682 5'UTR 80 6.1
CLIP2.060682_2 CLIP2.060682 5'UTR 80 6.1
CLIP2.069483_3 CLIP2.069483 3'UTR 80 6.1
CLIP2.069483_4 CLIP2.069483 3'UTR 80 6.1
CLIP2.073820_1 CLIP2.073820 3'UTR 80 6.1
CLIP2.073820_2 CLIP2.073820 3'UTR 80 6.1
CLIP2.079226_1 CLIP2.079226 3'UTR 80 6.1
LMJ.RY0402.070600_1 LMJ.RY0402.070600 3'UTR 95 5.5
LMJ.RY0402.070600_2 LMJ.RY0402.070600 3'UTR 95 5.5

Gene Annotations

PFAM:PF04774, PF09598
PANTHER: PTHR12299
KEGG_ec:EC:3.6.4.13
KEGG_orthology:K13199
KOG:KOG2945
Gene ontology terms::
Best arabidopsis TAIR name:AT4G16830
Best arabidopsis TAIR symbol:
Best arabidopsis TAIR defline:Hyaluronan / mRNA binding family
Phytozome Locus Page

Phenotypes

Our phenotype screens did not find statistically significant results for this gene.