| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071578 |
| Chromosome: | chromosome_5 |
| Location: | 2057293 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g234500 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGCGCATGTCGGCAGTCTGTGCCTCAAT |
| Internal bar code: | GTGGGGGGTGTGCTAGCCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 94.9747 |
| LEAP-Seq n confirming: | 203472 |
| LEAP-Seq n nonconfirming: | 10766 |
| LEAP-Seq n unique pos: | 188 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | GTTCAAAAAGCTCACGAGGC |