| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071727 |
| Chromosome: | chromosome_13 |
| Location: | 2671275 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g581850 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCTGTTCACCCACGATTGCCTACCGTAC |
| Internal bar code: | CGAGCACGGGTAAGCGGGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 90.4338 |
| LEAP-Seq n confirming: | 813 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTGTATATGTGTGGGAG |
| Suggested primer 2: | GCTGATGTGCAGCTTGTTGT |