Insertion junction: LMJ.RY0402.072149_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.072149
Chromosome:chromosome 3
Location:2604832
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g160650 (1 of 7) IPR013816 - ATP-grasp fold, subdomain 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGGCACCCATGAACCCACCACAGGAGC
Internal bar code:TCTGATCTCCCTAGACGTCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:83
LEAP-Seq percent confirming:61.3077
LEAP-Seq n confirming:797
LEAP-Seq n nonconfirming:503
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGTACGCGCACACAAGACAC
Suggested primer 2:TATGCTGCTGTCTGCTTGCT