| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072149 |
| Chromosome: | chromosome_12 |
| Location: | 3389614 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g497000 | FAP14 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCTGCAGGTTTTCACTGACTCACTGTTCG |
| Internal bar code: | TCACCCCAAGGCAAGCAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 99.5979 |
| LEAP-Seq n confirming: | 9165 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCTTGCTAGTGCCTCAT |
| Suggested primer 2: | GAACTTCCTTGTCCAGCAGC |