Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.072149 |
Chromosome: | chromosome_12 |
Location: | 3389623 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g497000 | FAP14 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGGAAATGGTTTCCGGCCTCAGTGCTTCAC |
Internal bar code: | CGTGGATGAAGATCTAGAGCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1150 |
LEAP-Seq percent confirming: | 99.7015 |
LEAP-Seq n confirming: | 2672 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCGCTTGCTAGTGCCTCAT |
Suggested primer 2: | GAACTTCCTTGTCCAGCAGC |