| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072481 |
| Chromosome: | chromosome 12 |
| Location: | 7579822 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555200 | (1 of 1) PF09786 - Cytochrome B561, N terminal (CytochromB561_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGTCGGCACCGGTGGCGTGGGCTCGC |
| Internal bar code: | GAGGTACGGAAATAGAAAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 26 |
| LEAP-Seq percent confirming: | 72.9644 |
| LEAP-Seq n confirming: | 1846 |
| LEAP-Seq n nonconfirming: | 684 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCACGCTCCTTGCTAAC |
| Suggested primer 2: | TAACGCTACAGGCGTACGTG |