| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072839 |
| Chromosome: | chromosome 12 |
| Location: | 866873 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495955 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACGCCGACCCTGGGTCATCACATTGGT |
| Internal bar code: | CCGGGGAGTCTGAATGCGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 74.6835 |
| LEAP-Seq n confirming: | 531 |
| LEAP-Seq n nonconfirming: | 180 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTAGGTTGCCATGACCT |
| Suggested primer 2: | TTGGAGGTTATCCACTTCGG |