| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072887 |
| Chromosome: | chromosome 10 |
| Location: | 1020200 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425050 | ROC59,ROC28 | (1 of 1) K11807 - WD and tetratricopeptide repeats protein 1 (WDTC1); Rhythm Of Chloroplast 59 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGAGCGGACGCATCCGATTTCCTTGACC |
| Internal bar code: | GTGCACTCTGGGTGCCACAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 98.2249 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTTGGTACGAGCACAGC |
| Suggested primer 2: | AAAGCAAGTTGGGAAGGGTT |